International Symposium on Bioorganic Chemistry (ISBOC-11) & Konstanz Symposium Chemical Biology

Restaurant Stromeyer - Die Bleiche,
Bleicherstrasse 8

8:00 - 9:00

Bioconjugation of Biopolymers, Bioorthogonal Chemistry | Andreas Marx

This talk highlighted and introduced the most important approaches for biorthogonal chemistry covering a large variety of chemistries.

Affinity Probes & Labeling Strategies – From Concepts to Applications | Thomas Böttcher

This talk focused on the application of bioconjugation chemistry for proteomic labeling strategies and protein profiling with chemical probes. Active site-directed probes targeting enzyme activities in complex proteomes have become an important tools for chemical biology. Hereby also various techniques for the cellular target profiling of small molecules have been discussed.

Small Molecule Screening | Thomas U. Mayer

The talk provided an overview of the spectrum of small molecule screening ranging from in vitro protein-based screens to high-content screens in cells. We highlighted the pros and cons of the different screening strategies and discussed optimal screening conditions using examples of screens performed in the in-house screening facility. A particular focus was on the challenge of target identification and specificity analyses of identified compounds. If desired, we were able to have a tour of the screening center.

Combinatorial Protein Design: Screening and Selection | Andreas Marx

This talk highlighted and introduced the most important approaches for evolving improved or new protein functions. Thereby, several screening and selection strategies were covered.

X-Ray Structure Analysis | Kay Diederichs

Knowledge of the structures of macromolecules is a prerequisite for understanding their functions and interactions. The talk presented important aspects of the theory and application of X-ray crystallography, the method that contributes about 90% of the macromolecular structures known today.

Biomolecular simulation | Christine Peter

The talk provided an introduction into classical molecular dynamics simulations. Such simulations provide a molecular view on biological systems and processes, they can be used to analyse the interplay of different types of relevant interactions, and they serve as a basis for a microscopic mechanistic interpretation of processes such as folding, binding, or aggregation.

Advanced (Life Science) Data Analysis | Michael Berthold

The talk provided an overview of classic and modern techniques for the analysis of various types of data: molecular databases, images, sequences, and mass spectrometry data. We showed how to process and integrate this type of data using the open source platform KNIME using extensions such as RDKit (chem informatics), SeqAn (NGS), FIJI (images), and OpenMS (mass spec).

12:00 - 13:00

IR-Spectroscopy | Karin Hauser

The course focused on IR-spectroscopy to study structure, function and dynamics of biomolecules ranging from small peptides to membrane proteins. Practical information (sample forms, sample preparation), various methods (FTIR, ATR, IR-laser spectroscopy) and aspects of data acquisition and analysis have been presented. Examples to study protein reaction mechanisms with time-resolved IR-spectroscopy have been given.

Fluorescence Techniques | Andreas Zumbusch

This part covered fluorescence techniques. After a short introduction to fluorescence spectroscopy, examples for application of fluorescence detection especially in the microscopy of biological samples have been given.