Laboratory of 
Analytical Chemistry and Biopolymer Structure Analysis

 

Dr Nikolay Youhnovski
Research Associate
Laboratory for Analytical Chemistry
Department of Chemistry
University of Konstanz

78457 Konstanz

 

Room L 847 / L 846
Ph.: +49 7531 88-2690 / +49 7531 88-2044
Fax: +49 7531 88-3097
E-mail: Nikolay.Youhnovski@uni-konstanz.de

 

Curriculum vitae

1966

born in Sofia, Bulgaria.

 

1986 - 1991

Study of organic chemistry and Diplomy thesis at the Higher Institute for Chemical Technology, Sofia, Bulgaria.
Synthesis and analysis of medicines.
Diploma thesis: "Synthesis and Characterisation of Sweet Peptides Consisting Cycloalkane Rings".

 

1991 - 1994

Scientific co-worker at Chemical and Pharmaceutical Research Institute, Sofia, Bulgaria. Subject: "Isolation and Analysis of Lovastatine".
Technology development: isolation and analysis of Lovastatine. The technology was implemented in practice at Antibiotics- Razgrad Ltd, Bulgaria.

 

1994 - 1999

PhD studies at the Zurich University, Switzerland.
PhD thesis: "On-line HPLC-MS Techniques for Structural Analysis of Complex Natural Extracts".
HPLC-MS and HPLC-MS/MS analysis, separation and structure elucidation of complex alkaloid extracts (HPLC, GC/MS, NMR, CD, IR).

 

1998 - 1999

Cantonal assistant for mass spectrometry at the Zürich University, Switzerland. Responsible for mass spectrometry service (internal and external contractors) at the institute with API methods.
LC-MS and LC-MS/MS (ESI and APCI) method development and analysis. Maintenance of mass spectrometers and HPLC.

 

1999 - 2000

Postdocoral fellowship. Work on the Deutsche Forschungs Gesellschaft (German Research Foundation) project at the University of Bayreuth, Germany.
Method development for isolation and mass spectrometric analysis of lipid peroxydation products in blood and cancer tissues. Used techniques: mass spectrometry (ESI, EI), HPLC, LC-MS, GC, and GC/MS. Supervision of technicians.

 

2001 to date

Head of Mass Spectrometry Laboratory for High-Resolution Biopolymer Mass Spectrometry at the University of Konstanz, Germany.
Laboratory work organisation, supervision of PhD students and technicians. Method development for protein and small molecules analysis using high resolution FT-ICR mass spectrometry and conventional MS: ESI, IR- and UV-MALDI, LC, LC-MS/MS. Chip-MS-Proteomics. Responsible for MS service for internal and external contractors.
Lectures and courses.
Maintenance of mass spectrometers and HPLC

 
Publications

  1. Youhnovski, N. , Bigler, L., Werner, Ch. and Hesse, M. On-Line Coupling of High Performance Liquid Chromatography to Atmospheric Pressure Chemical Ionization Mass Spectrometry (HPLC-APCI/MS and MS/MS). The Pollen Analysis of Hippeastrum x hortorum (Amaryllidaceae). Helv. Chim. Acta, 1998, 81, 1654.

  2. Youhnovski, N.,  Filipov, S.,  Linden, A.,  Guggisberg, A.,  Werner, Ch. and  Hesse, M. Two Macrocyclic Spermine Alkaloids from Aphelandra fuscopunctata (Acanthaceae). Phytochemistry, 1999, 52, 1717.

  3. Youhnovski, N.,   Drandarov, K.,  Guggisberg, A.and Hesse, M.  Macrocyclic Spermine Alkaloids from Verbascum: The (E/Z)-Isomeric Pairs (S)-Verbasikrine/(S)-Isoverbasikrine and (S)-Verbamekrine/(S)-Isoverbamekrine. Isolation, Structure Elucidation, and Synthesis. Helv. Chim. Acta‚ 1999, 82, 1185.

  4. Youhnovski, N., Werner, C.  and  Hesse, M. "N,N',N"-triferuloylspermidine, a new UV absorbing polyamine derivative from pollen of Hippeastrum x hortorum. Z Naturforsch [C], 2001, 56, 526-30.

  5. Przybylski, M., Youhnovski, N.,  Reymond, F.,  Rossier, J. 2001 UK Patent GB0121590.4 2001.

  6. Rossier, J.S.,Youhnovski, N., Lion, N., Damoc, E., Becker, S.,  Reymond, F.,  Girault, H., Przybylski, M. Thin-Chip Microspray System for High-Performance Fourier-Transform Ion-Cyclotron Resonance Mass Spectrometry of Biopolymers. Angew. Chemie Int. Ed, 2003, 42, 1, 53-58.

  7. Rossier, J. S.,  Youhnovski, N., Lion, N., Damoc, E., Becker, S.,  Reymond, F.,  Girault, H., Przybylski, M. Ein Chip-Mikrospraysystem für die hochauflösende Fourier-Transformations-Ionenzyklotronresonanz-Massenspektrometrie von Biopolymeren. Angew. Chemie, 2003, 115, 1, 55-60.

  8. Schmidt, P., Youhnovski, N., Daiber, A., Balan, A., Arsic, M.,  Bachschmid, M., Przybylski, M.  and  Ullrich, V. Specific nitration at tyrosine-430 revealed by high resolution mass spectrometry as basis for redox regulation of bovine prostacyclin synthase. J. Biol. Chem., 2003, 278, 15, 12813-9.
    On-line: http://www.jbc.org/cgi/reprint/M208080200v1.

  9. Damoc,E., Youhnovski, N., Crettaz,D., Tissot, J.-D. and  Przybylski, M. High Resolution Proteome Analysis of Cryoblobulins using Fourier Transform – Ion Cyclotron Resonance Mass Spectrometry. Proteomics, 2003, Aug; 3(8), 1425.