Laboratory of 
Analytical Chemistry and Biopolymer Structure Analysis

 

 
Dr Xiaodan Tian
Research Associate
Laboratory for Analytical Chemistry
Department of Chemistry
University of Konstanz

78457 Konstanz

 

Room L 834
Ph.: +49 7531 88-2285
Fax: +49 7531 88-3097
E-mail: Xiaodan.Tian@uni-konstanz.de

 

Curriculum vitae

1971

born in China.

 

1988 - 1992

Studies of chemistry (major Analytical Chemistry) at the Jilin University, China.
Bachelor thesis: "Studies of microwave plasma torch in the determination of rare-earth elements with ultrasonic nebulizer".

 

1992 - 1995

Master studies at the Faculty of Chemistry, Jilin University, China.
Master thesis: "Studies of helium direct current discharge ionization detector and microwave induced plasma ionization detector for gas chromatography".

 

1995 - 1999

PhD studies at the Department of Chemistry, Xiamen University, China.
 PhD thesis: "Application of the hyphenated technique of capillary electrophoresis and inductively coupled plasma atomic spectrometry/mass spectrometry in the element speciation analysis".

 

1999 - 2000

Post-doctoral Research Associate at the MiTAC, Department of Chemistry, Antwerp University (UIA), Belgium.
Project: Installation, analytical development of an inductively coupled plasma time of flight mass spectrometer (ICP-TOF-MS; first instrument in Europe) and studies of its application to isotope ratio analysis and coupling to high performance liquid chromatography.

 
2001 - 2003

Alexander von Humboldt Research Fellow; Post-doctoral Research Associate at the Department of Chemistry, Laboratory of Analytical Chemistry, University of Konstanz, Germany.
Project: Structure studies of target proteins in Alzheimer’s Disease and high resolution proteome analysis using Fourier transform ion cyclotron resonance (FTICR) mass spectrometry.

  

2003 to date

Habilitation in Laboratory of Analytical Chemistry, Department of Chemistry, University of Konstanz, Germany.
Project: High resolustion mass spectrometry in protein structure analysis and proteomics-method development and applications to neurodegenerative diseases.

 

Prizes

Prize for the best oral presentation at 7th International conference on plasma source mass spectrometry (Durham, England, September 2000).

Prize for the best poster at 2000 Winter Conference on Plasma Spectromchemistry (Fort Lauderdale, Florida, January 10-15, 2000).

Prize for the most outstanding students in the field of spectrometry (Xiamen, Feb.1998).

Prize for the best students in the field of analytical chemistry sponsored by Haiguang Foundation (Changchun, China, Jan. 1994)

Prize sponsored by Jilin University to “the best qualified student” (Changchun, Sept. 1990).

 
Research

Research work is focused on the development and application of functional proteomics methods to the studies of proteins and peptides in neurodegenerative diseases.

  
Publications

  1. Tian, X., Cecal, R., McLaurin, J., Manea M., Stefanescu, R., Grau, S., Harnasch, M., Amir, S., Ehrmann, M., St. George-Hyslop, P., Kohlmann, M. and Przybylski M. Characterisation of Target Polypeptides of Alzheimer's Disease and Specific Antibody Epitopes using High Resolution FTICR-MS. Eur. J. Mass Spectrom., 2004, in press.

  2. Grau, S., Baldi, A., Bussani, R., Tian, X., Stefanescu R., Przybylski, M., Steiner, H., Shridhar, V., Clausen, T., Ehrmann M. Implications of the Serine Protease HtrA1 in Amyloid Precursor Protein Processing. Cell, 2004, in press.

  3. Tian, X., Cecal, R., Mezo, G., Manea, M., Stefanescu, R., McLaurin, J., St.George-Hyslop, P., Hudecz, F., Przybylski, M. New amyloid-derived Vaccine Lead Structures against Alzheimer’s Disease Discovered by Affinity Proteomics and High Resolution Mass Spectrometry. In: Innovation and Perspectives in Antibody and Peptide Vaccine Development, (Ed. Epton, R.) Mayflower Worldwide Ltd, Kingswinford, UK, 2004, 79-82.

  4. Zirah, S., Stefanescu, R., Manea, M., Tian, X., Cecal, R., Kozin, S., Debey, P., Rebuffat, S., Przybylski, M. Zinc Binding Agonist Effect on the Recognition of the ß-amyloid (4-10) Epitope by anti-ß-amyloid Antibodies. Biochem. Biophy. Res. Comm., 2004, 321, 324-328.

  5. Przybylski, M., Tian, X., Cecal, R., Marquardt, A., Manea, M., Amstalden, E., Mezo, G., Hudecz, F., McLaurin, J. and St George-Hyslop, P. Affinity Proteomics and Mass Spectrometric Epitope Analysis: Powerful Tools for Identification of Vaccine Lead Structures against Alzheimer’s Disease. J Peptide Sci., 2004, 10, 296.

  6. Manea, M., Horvati, K. Mezo, G., Cecal, R., Tian, X., Stefanescu, R., Hudecz, F. and Przybylski, M. Synthesis, Antibody Recognition and Enzymatic Stability of Linear, Cyclic and Branched Polypeptides Containing a ß-amyloid Plaques Specific Epitope. J. Peptide Sci., 2004, 10, 291.

  7. McLaurin, J., Cecal, R., Kierstead, M., Tian, X., Phinney, A., Manea, M., French, J., Lambermon, M., Darabie, A., Brown, M., Janus, C., Chishti, M., Horne, P., Westaway, D., Fraser, P., Jmount, H., Przybylski, M., St. George-Hyslop, P. Sera Generated by Aß42-vaccination Recognise a Specific Epitope, Inhibit Aß- cytotoxiticity and fibrillogenesis, and Induce Fibril Disaggregation. Nature Med., 2002, 8, 1263-1269.

  8. Marquardt, A., Windberg, E., Tian, X., Kohlmann, M., Hudecz, F., Przybylski, M. Perspectives and Applications of Fourier Transform-ICR Mass Spectrometry for Vaccine Development and Analysis of Combinatorial Libraries. Proc. 6th Int. Sympos. SPPS & Combinator. Chem. Mayflower Sci. Publ. 2002, 89-92.

  9. Tian, X., Cecal, R., Amstalden, E., Kohlmann, M., Bruns, K., Bühler, S., Przybylski, M. Synthesis, Structural Characterisation and Epitope Elucidation of the Cytoplasmic Domain of Alzheimer’s Amyloid Precursor Protein (APP). J. Peptide Sci., 2002, 8, 182.

  10. Przybylski, M., McLaurin, J., Cecal, R., Kierstead, M. E., Tian, X., Janus, C., Horne, P., Westaway, D., Fraser, P. E., St George-Hyslop, P. Lead Structure for Active Immunisation against Alzheimer’s Disease (AD) upon Elucidation of a Plaque-specific Epitope Recognized by Therapeutically Active Antisera from Transgenic AD mice. J. Peptide Sci., 2002, 8, 73.

  11. Cecal, R., McLaurin, J., Kierstead, M. E., Tian, X., Janus, C., Horne, P., Westaway, D., Fraser, P. E., St George-Hyslop, P., Przybylski, M. Mass Spectrometric Elucidation of an Amyloid Plaque-specific Epitope: Molecular Basis for Probing the Proteolytic Degradation Mechanism of Alzheimer’s Amyloid Precursor Protein APP. J. Peptide Sci., 2002, 8, 81.

  12. Goenaga Infante, H., Heisterkamp, M., Van Campenhout, K., Tian, X., Blust, R., Adams, F. Time-of–Flight Inductively Coupled Plasma Mass Spectrometry for Ultratrace Speciation Analyisis of Organometallic Compounds. Plasma source mass spectrometry. The new Millennium, The Royal Chemical Society, Cambridge, UK. 2001. 369-379.

  13. Tian, X., Emteborg, H., Barbaste, M. and Adams, F. Accuracy and Precision of Lead Isotope Ratios in Wines Measured by Axial Inductively Coupled Plasma Time of Flight Mass Spectrometry. J. Anal. Atom. Spectrom., 2000, 15, 829-835.

  14. Emteborg, H., Tian, X., Ostermann, M., Berglund, M., Adams, F. Isotope Ratio and Isotope Dilution Measurements using Axial Inductively Coupled Plasma Time of Flight Mass Spectrometry. J. Anal. Atom. Spectrom., 2000, 15, 239-246.

  15. Adams, F., Tian, X., Heisterkamp M. ICP time-of-flight Mass Spectrometry for Elemental and Speciation Analysis of Environmental Samples. Proceedings of the Third Biennial International Conference on Monitoring and Measurement of the Environment / Clement R. [edit.], e.a., Ottawa, Ont., s.n., 2000, 131-136.

  16. Emteborg, H., Tian, X., Heisterkamp, M., Adams, F. Inductively Coupled Plasma Time of Flight Mass Spectrometry for Trace Element and Speciation Analysis. Trace elements in man and animals 10 / Roussel A.M. [edit.], e.a., New York, Kluwer, 2000, 1107-1110.

  17. Tian, X., Emteborg, H. and Adams, F. Analytical Performance of Axial Inductively Coupled Plasma Time of Flight Mass Spectrometry (ICP-TOF-MS). J. Anal. Atom. Spectrom., 1999, 14, 1807-1814.

  18. Emteborg, H., Tian, X. and Adams, F. Quality Assurance of Arsenic, Lead, Tin and Zinc in Copper Alloys using Axial Inductively Coupled Plasma Time-of-Flight Mass Spectrometry (ICP-TOF-MS). J. Anal. Atom. Spectrom., 1999, 14, 1567-1572.

  19. Tian, X., Emteborg, H., Berglund, M., Adams, F. Application of inductively coupled plasma time of flight mass spectrometry (ICP-TOF-MS) for elemental analysis. Proceedings International Conference Instrumental Methods of Analysis, Modern Trends and Applications: volume 1 / Ochsenkühn-Petropulu M. [edit.], e.a., Athens, University of Athens, 1999, 165-168.

  20. Adams, F., Emteborg, H., Tian, X. and Heisterkamp, M. Inductively Coupled Plasma Time of Flight Mass Spectrometry, ICP-TOF-MS for Isotope Ratio, Elemental and Speciation Analysis. Proceedings International Conference Instrumental Methods of Analysis, Modern Trends and Applications: volume 1 / Ochsenkühn-Petropulu M. [edit.], e.a., Athens, University of Athens, 1999, 31-38.

  21. Tian, X., Zhuang, Z., Chen, B. and Wang, X. Analysis of Arsenic by Hyphenation of Capillary Electrophoresis (CE) and ICP-MS using a Movable Reduction Bed Hydride Generation System. Atomic Spectr., 1999, 20(4), 127-134.

  22. Tian, X., Zhuang, Z., Chen, B. and Wang, X. Movable Reduction Bed Generation System as an Interface for Capillary Zone Electrophoresis and Inductively Coupled Plasma Atomic Emission Spectrometry for Arsenic Speciation Analysis. The Analyst, 1998, 123, 899-903.

  23. Tian, X., Zhuang, Z., Chen, B. and Wang, X. Movable Reduction Bed Hydride Generator Coupled with Inductively Coupled Plasma Atomic Emission Spectrometry for the Determination of Some Hydride Forming Elements. The Analyst, 1998, 123, 627-632.

  24. Zhuang, Z., Tian, X., Chen, B. and Wang, X. A Novel Hydride Generation Technique-Movable Reduction Bed Hydride Generator (MRBHG)-1. The Configuration and Characteristics of the Movable Reduction Bed Hydride Generator. Yan Kuang Ce Shi, 1999, 18(1), 25-28.

  25. Tian, X., Zhuang, Z., Chen, B. and Wang, X. A Novel Hydride Generation Technique-Movable Reduction Bed Hydride Generator (MRBHG)-2. Study of interferences and applications. Yan Kuang Ce Shi, 1999, 18(2), 87-91.

  26. Tian, X., Zhuang, Z., Chen, B. and Wang, X. A Novel Hydride Generation Technique-Movable Reduction Bed Hydride Generator (MRBHG)-3. As an interface between capillary zone electrophoresis (CZE) and inductively coupled plasma atomic emission spectrometry (ICP-AES) for arsenic speciation analysis. Yan Kuang Ce Shi, 1999, 18(3), 165-170.

  27. Tian, X., Yang, P., He, X. and Wang, X. Application of Capillary Zone Electrophoresis in Arsenic Speciation Analysis. Chem. J. Chin. Univ., 1998, 1, 27-30.

  28. Jin, Q., Yang, W., Yu, A., Tian, X. and Wang, F. Helium Direct Current Discharge Ionization Detector for Gas Chromatography. J. Chrom. A., 1997, 761, 169-179.

  29. Tian, X., Yang, P. and Wang, X. Studies on Speciation Analysis of Arsenic by Capillary Zone Electrophoresis. Adv. Anal. Chem., 1997, 827-828.

  30. Tian, X., Yang, W., Liu, M., Yu, A. and Jin, Q. Studies on Helium Direct Current Plasma Ionization Detector for Gas Chromatography. Acta Chimica Sinica, 1996, 54, 691-696.

  31. Tian, X., Yang, W., Yu, A. and Jin, Q. Studies on a Helium Ionization Detector for Gas Chromatography. Microchem. J., 1995, 52, 139-145.

  32. Tian, X., Yang, W., Yu, A. and Jian, Q. Studies on Direct Current Plasma Ionization Detector-Measurements of Flammable Gas in Coal Gas. J. Anal. Sci., 1995, 11(2), 32-34.

  33. Tian, X., Yang, W., Yu, A. and Jian, Q. Determination of Carbon Dioxide in Air by Novel Direct Current Plasma Ionization Detector. Acta Sci. Nat. (Chinese), 1995, 2, 83-86.

  34. Duan, Y., Li, Y., Tian, X., Zhang, H. and Jin, Q. Analytical Performance of the Microwave Plasma Torch in the Determination of Rare-Earth Elements with Optical Emission Spectrometry. Anal. Chim.  Acta, 1994, 295, 315-324.