Analytical Chemistry Laboratory - FTICR-MS
February 2009
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Recent Results and Publications
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"Unknown genome" proteomics: a new NADP-dependent epimerase/dehydratase revealed by
N-terminal sequencing, inverted PCR, and high resolution mass spectrometry.
(Simeonova DD, Susnea I et al.,
Mol Cell Proteomics 2009
8(1), 122-131)
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Elucidation of O-Glycosylation Structures of the ß-Amyloid Precursor
Protein by Liquid Chromatography-Mass Spectrometry Using
Electron Transfer Dissociation and Collision Induced Dissociation
(Perdivara I. et al., J. Proteome Res
8 (2) 631-642
Three specific O-glycosylation sites of the secreted APP695 (sAPP695)
produced in CHO cells have been identified using a combination of high-performance liquid
chromatography and electrospray-tandem mass spectrometry,
with electron transfer
dissociation and collision induced dissociation (ETD and CID).
The glycosylations comprise multiple short glycans, containing N-acetyl galactosamine (GalNAc),
Gal-GalNAc and sialic acid terminated structures.
The combination of the CID and ETD techniques in LC-MS is shown as a powerful
tool for de novo identification of O-glycosylations at unknown modification
sites in proteins.
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Binding Epitopes and Interaction Structure of the Neuroprotective Protease Inhibitor Cystatin
C with ß-Amyloid Revealed by Proteolytic Excision Mass Spectrometry and Molecular Docking
Simulation
(Juszczyk P. et al.,
J. Med. Chem. 2009
52, 2420–2428)
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Aberrant processing forms of lung surfactant proteins SP-B and SP-C revealed by high resolution mass spectrometry
(Galetskiy D. et al., Eur J Mass Spectrom
in press,
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Structure and dynamics of photosystem II light-harvesting complex revealed by high-resolution FTICR mass spectrometric proteome analysis
(Galetskiy D. et al.,
J Am Soc Mass Spectrom 2008
19 (7), 1004-13)
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Copper-induced
oligomerization of peptides: a model study
(G. Schlosser et
al., Eur. J. Mass
Spectrom. 2007,13(5),331-7) |
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"Peptidbody" Design:
Functional single-chain peptide anti-lysozyme antibody,
revealed by high-resolution affinity - mass spectrometry
(A. Marquardt et al., Chemistry 2006 12(7): 1915-1923
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Identification of
Tyrosin-nitration in proteins by high resolution
IR-MALDI-FTICR-MS
(A. Petre et al., Eur. J. Mass Spectrom 2005 11(5): 513-518
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Proteolytic specificity
of serine protease HtrA1 & implications for amyloid
precursor protein (APP) processing revealed by
FTICR-MS
(S. Grau et
al., Proc Natl Acad Sci USA 2005,
102(17),
6021) |
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Oxidative protein
modification: Inactivation of prostacyclin synthase by
nitration of active-site Tyr-430 residue
(P. Schmidt et al., J. Biol. Chem. 2003, 278,
12813)
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Elucidation of a
plaque-specific epitope β-amyloid (4-10) provides
Alzheimer-vaccine lead structure
(J. McLaurin et al., Nature Med 2002, 8, 1263;
M.
Manea
et
al., Biopolymers 2004, 76,
503) |
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