Bioinformatics Lectures
Previous Lecture 9th Lecture Summer Course - Functional Genomics II: Protein Interactions Next Lecture
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  1. Functional Genomics II  - Protein Interactions
  2. Dissecting Cellular Pathways II - Protein Interactions
  3. Protein - Protein Interaction Profiling
  4. Protein Interactions
  5. Experimental System to Determine Protein Interactions
  6. Y2H Components - GAL4 Transcription Factor Protein
  7. Y2H Components -  LexA-based 2-Hybrid Systems
  8. Modular Structure of Eukaryotic Transcription Factors
  9. Y2H - Split Domains and construct Fused Peptides
  10. Y2H - Defining Bait and Prey
  11. Y2H - The Test System Uses a Reporter Gene
  12. The Yeast-2-Hybrid System  (Y2H) Components
  13. Y2H Bait & Prey Cloning Vectors
  14. Y2H - The Complete System
  15. Y2H Workflow  I
  16. The Y2H Workflow  II
  17. Y2H - Analysis of Protein Complexes
  18. A Dual Reporter System
  19. Variants of the Y2H System - Reverse Testing
  1. Using the Reverse Yeast-One-Hybrid System (Y1H)
  2. An Interaction Map of the RNA Polymerase III Complex
  3. System  II - The Split Ubiqitin System
  4. Split Ubiquitin -  A Flexible Screening System
  5. Principles of Split Ubiquitin Assay Integration with Y2H System Components
  6. Measuring Transmembrane Protein Interactions with the Split Ubiquitin System
  7. Generation of Interaction Libraries
  8. Analysis of Interaction Libraries
  9. A Y2H Protein Interaction Profile
  10. Identification of True Positives
  11. Yeast-Based Split Ubiquitin Interaction Screens
  12. Split Ubiquitin System -  LacZ Interaction Detection
  13. A Protein Interaction Map of the Urease Operon in Helicobacter
  14. Y2H System - Be Aware of the Pitfalls
  15. Roboterization Allows Large Scale Parallel Screening
  16. Screening Modalities for Large Scale Parallel Screening
 ©    Klaus P. Schäfer - Last Update  13.05.2003